scWGA data (hg38 DNA reads on chr17)

We used R9.4.1 reads from both whole genome shotgun (WGS) and single-cell whole genome amplification (scWGA) sequencing results for current events visualization. In theory, scWGA reads were generated from amplified DNA without 5mC signals and can thus be used as a negative control for WGS reads. Reads aligned around the site 43281378 on chr7 are sampled to showcase. scWGA data is used as the negative control.

本研究采用全基因组鸟枪测序（whole genome shotgun, WGS）与单细胞全基因组扩增（single-cell whole genome amplification, scWGA）测序产出的R9.4.1版本测序读段开展电流事件可视化分析。理论上，scWGA测序读段源自未携带5-甲基胞嘧啶（5mC）修饰的扩增DNA，因此可作为WGS测序读段的阴性对照。我们选取比对至7号染色体（chr7）上43281378位点附近的测序读段作为示例展示，其中scWGA数据集即作为该阴性对照。