Proteogam02_Supplementary File 2_Translated ORFs.zip

We sequenced the transcriptomes of a male individual and a female individual for seven different taxonomical groups: Gammarus fossarum A, G. fossarum B, G. fossarum C, Gammarus wautieri, Gammarus pulex, Echinogammarus berilloni and Echinogammarus marinus. Translations of coding sequence regions were produced for each transcriptome by Transdecoder from stop to stop codons, analysing only top strand with 500 top longest ORFs used for training, and retaining 600 base pair ORFs and only proteins with a minimum length of 50 amino acids. <br>

本研究对7个不同分类群的雌雄个体开展转录组测序，涉及的分类群依次为：Gammarus fossarum A、G. fossarum B、G. fossarum C、Gammarus wautieri、Gammarus pulex、Echinogammarus berilloni及Echinogammarus marinus。
针对每个转录组，本研究通过Transdecoder工具以终止密码子为边界对编码序列区域进行翻译；分析时仅选取正义链，筛选出长度排名前500的最长开放阅读框（Open Reading Frame, ORF）用于模型训练，同时保留长度≥600碱基对的开放阅读框，且仅保留氨基酸最小长度为50的蛋白质序列。