Single cell RNA sequencing of rat lung tissues to characterize blast exposure-induced cell heterogeneity. Single cell RNA sequencing of rat lung tissues to characterize blast exposure-induced cell heterogeneity

Coal mining carries inherent risks of catastrophic gas explosions capable of inflicting severe lung injury. Using a rat model, we explored mechanisms underlying alveolar epithelial damage and repair following a gas explosion. By performing scRNA-sequencing, we revealed that alveolar epithelial cells exhibited the most profound transcriptomic changes after gas explosion compared to other pulmonary cell types. Overall design: To investigate the variation of lung cells, we exposed rats to blast explosion and matched them with control. The lung tissues of both groups were subjected to scRNA-sequencing.

煤矿开采存在固有风险，即灾难性瓦斯爆炸，可引发严重肺损伤。本研究以大鼠为模型，探究瓦斯爆炸后肺泡上皮损伤与修复的潜在机制。通过单细胞RNA测序(scRNA-sequencing)，我们发现相较于其他肺细胞类型，瓦斯爆炸暴露后肺泡上皮细胞的转录组变化最为显著。实验设计：为探究肺细胞的转录组差异，我们将大鼠暴露于爆炸环境，并设置匹配对照组。两组大鼠的肺组织均接受单细胞RNA测序分析。