Expression studies of HMEC exposed to lactic acidosis and hypoxia

Human Mammalian Epithelial Cells (HMEC) were exposed to different environmental stresses, including hypoxia, lactic acidosis, the combination of hypoxia and lactic acidosis, lactosis , as well as acidosis. We used microarrays to examine the genomic programs of cells incubated under different microenvironments. Keywords: different environmental stresses HMEC cells were exposed to different environmental stresses and RNAs were extracted and put on Affymetrix microarrays. We gathered RNAs from cells grown in regular media (control), lactic acidosis, hypoxia, the combinatino of lactic acidosis and hypoxia, lactosis, as well as acidosis.

将人源哺乳动物上皮细胞（Human Mammalian Epithelial Cells, HMEC）暴露于多种不同环境胁迫，包括缺氧、乳酸酸中毒、缺氧与乳酸酸中毒联合处理、乳酸血症以及酸中毒。我们采用微阵列技术，分析不同微环境下培养细胞的基因组表达程序。
关键词：不同环境胁迫
本研究将HMEC细胞暴露于不同环境胁迫，提取细胞RNA并加载至Affymetrix微阵列芯片中。我们收集了常规培养基培养（对照组）、乳酸酸中毒、缺氧、乳酸酸中毒与缺氧联合处理、乳酸血症以及酸中毒环境下生长细胞的RNA。