PRMT-7/PRMT7 activates HLH-30/TFEB to guard plasma membrane integrity compromised by pore-forming toxins

This research reveals that protein arginine methyltransferase-7 (PRMT-7) is indispensable to the nuclear transactivation of HLH-30. We demonstrated that PRMT-7 participates in the methylation of HLH-30 on its Rag complex binding domain to facilitate its nuclear localization. Moreover, we showed that PRMT7 is evolutionarily conserved to regulate TFEB cellular localization and repair plasma damage caused by PFTs in human intestinal cells.

本研究揭示，蛋白质精氨酸甲基转移酶7（protein arginine methyltransferase-7, PRMT-7）是HLH-30核反式激活过程所不可或缺的。我们证实，PRMT-7可对HLH-30的Rag复合物结合域进行甲基化修饰，进而促进其核定位。此外，我们还发现，PRMT7在进化上具有保守性，其能够调控TFEB的细胞定位，并修复穿孔毒素（pore-forming toxins, PFTs）所致的人肠道细胞的细胞膜损伤。