Transcriptome of primary KP lung adenocarcinoma cells with Nupr1 knockout

The aim for this bulk mRNA sequencing project is to understand the age-specific impact of Nupr1 suppression in lung adenocarcinoma by CRISPR-Cas9 mediated gene knockout in vivo. Lung adenocarcinomas were induced in aged (> 2 years old) vs young (12-16 weeks old) Kras LSL-G12D/+; Trp53 fl/fl; Rosa26-LSL-EGFP-Cas9 (KPCas9) mice. Mice were intubated with lentivirally delivered Cre recombinase, together with a control or two sgRNAs targeting murine Nupr1. Ten weeks after tumor induction, GFP positive tumor cells were sorted by FACS from both aged and young tumor-bearing mice and subjected to mRNA sequencing.

本批量mRNA测序（bulk mRNA sequencing）项目旨在通过CRISPR-Cas9介导的体内基因敲除，探究Nupr1抑制在肺腺癌中的年龄特异性影响。本研究分别于老年（年龄＞2岁）与年轻（12~16周龄）的Kras LSL-G12D/+；Trp53 fl/fl；Rosa26-LSL-EGFP-Cas9（简称KPCas9）小鼠模型中诱导肺腺癌。通过气管插管向小鼠递送慢病毒包装的Cre重组酶（Cre recombinase），同时辅以靶向小鼠Nupr1的单向导RNA（sgRNA）对照或两条靶向sgRNA。肿瘤诱导10周后，通过荧光激活细胞分选（FACS）从老年和年轻的荷瘤小鼠中分离绿色荧光蛋白（GFP）阳性肿瘤细胞，并进行批量mRNA测序。