Rhodococcus qingshengii strain:RL1 | isolate:RL1 Genome sequencing. Rhodococcus qingshengii strain:RL1 | isolate:RL1

With an aim to isolate endophytes from leafy greens which are consumed raw, we examined the plant Rucola (Eruca sativa), belonging to the family Brassicaceae. The members of this family produce glucosinolates, nitrogen- and sulfur-containing secondary metabolites. We sequenced the complete genome using Single-molecule real-time (SMRT) sequencing (Pacific Biosystems). Genes previously identified for the biotransformation of glucosinolates (methionine sulphoxide reductase, msrA, msrB) were identified from the draft genome. The in silico analyses further revealed presence of several antibiotics and secondary metabolite genes as well as traits commonly associated with plant-bacteria interactions and potential biocontrol ability. Ongoing annotation of complete genome and high throughput mass spectrometric analyses to follow the metabolism of glucosinolates would reveal more information about the genotypic and phenotypic properties of the isolate.

本研究旨在从可生食叶菜中分离内生菌，故而选取隶属于十字花科（Brassicaceae）的芝麻菜（Eruca sativa，俗称Rucola）作为研究对象。该科植物均可合成硫代葡萄糖苷（glucosinolates）——一类含氮、硫的次生代谢产物。本研究采用单分子实时（Single-molecule real-time, SMRT）测序技术（太平洋生物科学公司（Pacific Biosystems）平台）完成了该菌株的全基因组测序。从该菌株的草图基因组中，我们成功鉴定出此前已被证实可介导硫代葡萄糖苷生物转化的基因：甲硫氨酸亚砜还原酶（methionine sulphoxide reductase）msrA与msrB。后续虚拟计算分析进一步显示，该菌株携带多种抗生素合成相关基因与次生代谢产物编码基因，同时具备植物-细菌互作相关的典型性状及潜在生防能力。当前，本团队正持续推进该全基因组的注释工作，并将通过高通量质谱分析追踪硫代葡萄糖苷的代谢通路，相关研究结果将进一步揭示该分离菌株的基因型与表型特性。