Mass Spectrometry analysis of N-glycans from resting and activated Tregs

N-glycans from the Tregs were permethylated and analysed by MALDI-TOF mass spectrometry. The data were acquired in the form of [M+Na]+ ions. Peaks representing hybrid and complex glycans were annotated according to the molecular weight and N-glycan biosynthetic pathways. <br>来自调节性T细胞（Regulatory T cells，Tregs）的N-聚糖（N-glycans）经全甲基化处理后，采用基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱进行分析。实验数据以[M+Na]+离子形式采集获得。代表杂合型与复合型聚糖的质谱峰，依据分子量及N-聚糖生物合成途径完成注释。