Effect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements

Abstract Objective: This study evaluated the angiogenesis-enhancing potential of a tricalcium silicate-based mineral trioxide aggregate (ProRoot MTA), Biodentine, and a novel bioceramic root canal sealer (Well-Root ST) in human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPLSCs), and human tooth germ stem cells (hTGSCs). Methodology: Dulbecco's modified Eagle's medium was conditioned for 24 h by exposure to ProRoot MTA, Biodentine, or Well-Root ST specimens (prepared according to the manufacturers’ instructions). The cells were cultured in these conditioned media and their viability was assessed with 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H tetrazolium (MTS) on days 1, 3, 7, 10, and 14. Angiogenic growth factors [platelet-derived growth factor (PDGF), basic fibroblast growth factor (FGF-2), and vascular endothelial growth factor (VEGF)] were assayed by sandwich enzyme-linked immunosorbent assay (ELISA) on days 1, 7, and 14. Human umbilical vein endothelial cell (HUVEC) migration assays were used to evaluate the vascular effects of the tested materials at 6–8 h. Statistical analyses included Kruskal–Wallis, Mann–Whitney U, and Friedman and Wilcoxon signed rank tests. Results: None of tricalcium silicate-based materials were cytotoxic and all induced a similar release of angiogenic growth factors (PDGF, FGF-2, and VEGF) (p>0.05). The best cell viability was observed for hDPSCs (p

研究目的：本研究评估了硅酸三钙基矿物三氧化物凝聚体ProRoot MTA、Biodentine以及新型生物陶瓷根管封闭剂Well-Root ST，在人牙髓干细胞（human dental pulp stem cells, hDPSCs）、人牙周膜干细胞（human periodontal ligament stem cells, hPLSCs）和人牙囊干细胞（human tooth germ stem cells, hTGSCs）中的促血管生成潜力。
研究方法：将ProRoot MTA、Biodentine或Well-Root ST标本按照厂商说明书制备后，置于杜氏改良伊格尔培养基（Dulbecco's modified Eagle's medium, DMEM）中孵育24小时以制备条件培养基。将细胞接种于上述条件培养基中培养，并分别于第1、3、7、10和14天采用3-(4,5-二甲基噻唑-2-基)-5-(3-羧基甲氧基苯基)-2-(4-磺基苯基)-2H四唑鎓盐（3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H tetrazolium, MTS）法检测细胞活力。分别于第1、7和14天采用夹心酶联免疫吸附试验（sandwich enzyme-linked immunosorbent assay, ELISA）检测血管生成生长因子：血小板衍生生长因子（platelet-derived growth factor, PDGF）、碱性成纤维细胞生长因子（basic fibroblast growth factor, FGF-2）与血管内皮生长因子（vascular endothelial growth factor, VEGF）的表达水平。于6~8小时时采用人脐静脉内皮细胞（human umbilical vein endothelial cell, HUVEC）迁移实验评估受试材料的血管生成效应。统计学分析采用Kruskal-Wallis检验、Mann-Whitney U检验、Friedman检验及Wilcoxon符号秩检验。
研究结果：所有硅酸三钙基材料均无细胞毒性，且均可诱导相似水平的血管生成生长因子（PDGF、FGF-2和VEGF）释放（p>0.05）。其中人牙髓干细胞的细胞活力最佳（p