Subcellular localization of KIF20A in porcine oocytes.

Immunofluorescent staining was employed to show the subcellular localization of KIF20A. KIF20A exhibited a dynamic localization pattern during porcine oocyte meiotic maturation. KIF20A localized at centromeres at the germinal vesicle breakdown (GVBD) stage and metaphase I (MI) stage, then transferred to the midbody in dividing oocytes during telophase (TI), finally, KIF20A was accumulated at centromeres again at metaphase II (MII). Red, KIF20A; blue, chromatin.

本实验采用免疫荧光染色技术，可视化展示驱动蛋白家族成员20A（KIF20A）的亚细胞定位。在猪卵母细胞减数分裂成熟进程中，KIF20A展现出动态的定位模式：在生发泡破裂（germinal vesicle breakdown, GVBD）时期与减数分裂I中期（metaphase I, MI），KIF20A定位于着丝粒；随后在处于减数分裂末期（telophase, TI）的分裂卵母细胞中，其定位转移至分裂中体；最终在减数分裂II中期（metaphase II, MII），KIF20A再次富集于着丝粒。红色荧光信号标记KIF20A，蓝色荧光信号标记染色质。