Genome-wide DNA methylation analysis in permanent atrial fibrillation. Homo sapiens

Atrial fibrillation (AF) is the most common sustained arrhythmia with increased risk of stroke and congestive heart failure. AF is a highly genetic heterogeneous disease, but a large proportion of AF cannot be explained by genetic variants only. Some risk factors of AF, tendentious heritable phenomenon, potentially reversible conditions and some subtypes without DNA sequence variation all indicate the participation of DNA methylation in the pathogenesis of AF. Overall design: Bisulphite converted DNA from the 11 left atrium samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip GPL13534

心房颤动（Atrial Fibrillation，AF）是最常见的持续性心律失常，患者罹患中风与充血性心力衰竭的风险显著升高。AF属于高度遗传异质性疾病，但绝大多数AF的发病机制无法仅通过遗传变异予以解释。部分AF的危险因素、倾向性可遗传现象、潜在可逆的病理状态，以及若干不存在DNA序列变异的AF亚型，均提示DNA甲基化参与了AF的发病过程。总体实验设计：对11例左心房样本的亚硫酸氢盐转化DNA，采用Illumina Infinium 450k人类甲基化微珠芯片（GPL13534）进行杂交检测。