Effect of sc3 and sc4 on aerial hypha formation. Schizophyllum commune

In order to study changes in gene expression during mushroom development in Schizophyllum commune, genome wide gene expression was analysed in 4 developmental stages: vegetative mycelium, stage I aggregates, stage II primordia and mature mushrooms. In order to study the effects of absence of expression of sc3 and sc4, genome-wide expression was analysed in a dikaryon in which those genes have been inactivated by targeted gene deletion. Overall design: 5 samples: - vegetative mycelium - stage I aggregates - stage II primordia - mature mushrooms - deltaSC3 deltaSC4 dikaryon RNA was obtained from 3 biological replicates and pooled

为研究裂褶菌（Schizophyllum commune）发育过程中的基因表达变化，研究人员对4个发育阶段的样本开展了全基因组基因表达分析，分别为营养菌丝体、I级聚集体、II级原基以及成熟子实体。为探究sc3与sc4基因表达缺失的影响，研究人员对通过靶向基因敲除技术使上述基因失活的双核菌株进行了全基因组表达分析。整体实验设计：共包含5个样本：
- 营养菌丝体
- I级聚集体
- II级原基
- 成熟子实体
- ΔSC3 ΔSC4双核菌株
所有样本的RNA均取自3次生物学重复，并经混合处理。