Tissue macrophage subsets derived from regenerating muscle

Muscle injury was elicited by cardiotoxin injection into the tibialis anterior muscle. Macrophages were isolated 2 days post-injury from the regenerating muscle. We used microarray to obtain global gene expression data of muscle-derived tissue macrophage subsets. Tissue macrophages were collected from regenerating muscle samples of three animals, Ly6C+ F4/80low and Ly6C- F4/80high macrophage subsets were sorted. The global gene expression patterns of distinct macrophage subsets were analyzed on Affymetrix microarrays.

向胫前肌（tibialis anterior muscle）注射心脏毒素（cardiotoxin）诱导肌肉损伤。于损伤后2天从再生肌肉组织中分离巨噬细胞。本研究采用基因芯片（microarray）获取肌肉来源组织巨噬细胞亚群的全基因组表达谱数据。从3只实验动物的再生肌肉样本中收集组织巨噬细胞，并分选得到Ly6C+ F4/80low与Ly6C- F4/80high两种巨噬细胞亚群。采用Affymetrix基因芯片分析不同巨噬细胞亚群的全基因表达模式。