Alternate genome assemblies of Halicephalobus mephisto

Alternate genome assemblies were generated using Illumina data from a published study (Weinstein et al, 2019) publicly available on Sequence Reads Archive (SRA) at accession PRJNA528747. Assemblies were generated with either Platanus or SoapDenovo2 using varying step-sizes (Platanus) or kmers (SoapDenovo2) and using either a 1kb or 200bp cutoff on scaffold lengths. These assemblies were then analyzed in a separate study (under review) to identify regional expansions or contractions associated with high levels of heterozygosity. Cited: Weinstein, D.J., Allen, S.E., Lau, M.C.Y. <em>et al.</em> The genome of a subterrestrial nematode reveals adaptations to heat. <em>Nat Commun</em> <strong>10, </strong>5268 (2019). https://doi.org/10.1038/s41467-019-13245-8本研究采用已发表研究（Weinstein等，2019）中的Illumina测序数据，构建了多套替代基因组组装结果，该数据已通过序列读取档案库（Sequence Reads Archive，SRA）以登录号PRJNA528747公开获取。 组装过程分别使用Platanus与SoapDenovo2两款软件完成：针对Platanus设置不同步长参数，针对SoapDenovo2设置不同k-mer参数；同时对序列支架（scaffold）长度设置1kb或200bp的截断阈值。 随后，另有一项在审研究对上述组装结果进行分析，以鉴定与高水平杂合性相关的基因组区域扩张与收缩事件。 引用文献：Weinstein, D.J.、Allen, S.E.、Lau, M.C.Y. 等. 地下线虫基因组揭示其对高温的适应性. *Nat Commun*，10卷，5268（2019）. https://doi.org/10.1038/s41467-019-13245-8