SH1399891.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于界定、识别、交流及处理基于 DNA 的物种假说（Species Hypotheses, SH）。国际核酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer, ITS）序列，均通过应用一组动态距离值（<0.5% - 3.0%）聚类至近似物种级水平。所有物种假说均被赋予唯一且稳定的数字对象唯一标识符（Digital Object Identifier, DOI），其分类学与生态学注释将通过分布式、基于网页的第三方注释工作完成验证。物种假说会尽可能关联至对应分类单元的名称及其分类层级（门、纲、目等），该过程会综合考量该物种假说下所有序列的鉴定结果。研究人员会为每个物种假说选取一条自动或人工指定的序列作为代表序列。此类代表序列已通过官方仓库（https://unite.ut.ee/repository.php）对外发布，可供科学界用于本地序列相似性检索、下一代测序分析流程等研究场景。随着公共真菌 ITS 序列数量的持续增长，该系统与配套数据集将自动完成更新。