Table_1_Cyclooxygenase-2 and β-Catenin as Potential Diagnostic and Prognostic Markers in Endometrial Cancer.docx

Objectives: Explore the diagnostic and prognostic value of cyclooxygenase-2 and wnt3a/β-catenin pathway in endometrial cancer.
Methods: A prospective cohort study of 93 women underwent hysterectomy at the China-Japan Friendship Hospital (61 patients with primary endometrial carcinoma, and 32 control patients with uterine prolapse or leiomyoma of uterus). Cox2 and β-catenin expression were determined by immunohistochemistry. The serum levels of cox2 and wnt3a were detected via ELISA assays.
Results: Patients with endometrial cancer showed overexpression of cox2 and β-catenin, as well as significantly higher serum levels of cox2 and wnt3a. The serum cox2 level, which is highly significant in predicting the risk of disease progression (RR, 9.617, 95% confidence interval, 1.162–79.622, P = 0.036), showed good diagnostic and prognostic potential, with cut-off of 55 U/L, but alongside β-catenin expression in tissues, were related to poor prognosis (RR, 12.426; 95% confidence interval, 1.618–95.450; P = 0.015).
Conclusion: Serum levels of cox2 and wnt3a exhibited diagnostic value for endometrial cancer. Cox2 serum levels and β-catenin expression also showed potential value of prognostic prediction. Cox2 serum levels might be a potential marker for early diagnosis and prognosis prediction in endometrial cancer.

研究目的：探讨环氧合酶-2（cyclooxygenase-2）及wnt3a/β-连环蛋白（β-catenin）通路在子宫内膜癌中的诊断与预后价值。
研究方法：本研究为前瞻性队列研究，纳入中日友好医院行子宫切除术的93例女性患者，其中61例为原发性子宫内膜癌患者，32例为对照患者（罹患子宫脱垂或子宫平滑肌瘤）。采用免疫组织化学法检测组织中环氧合酶-2与β-连环蛋白的表达水平；通过酶联免疫吸附试验（ELISA）检测血清中环氧合酶-2及wnt3a的水平。
研究结果：子宫内膜癌患者的环氧合酶-2与β-连环蛋白呈过表达状态，且血清环氧合酶-2及wnt3a水平显著升高。血清环氧合酶-2水平对疾病进展风险具有显著预测价值（风险比RR=9.617，95%置信区间1.162~79.622，P=0.036），以55U/L作为截断值时，其具备良好的诊断及预后潜能；而组织β-连环蛋白的表达与不良预后密切相关（RR=12.426，95%置信区间1.618~95.450，P=0.015）。
研究结论：血清环氧合酶-2及wnt3a水平对子宫内膜癌具有诊断价值。血清环氧合酶-2水平与组织β-连环蛋白表达同样具备预后预测潜力。血清环氧合酶-2水平或可作为子宫内膜癌早期诊断及预后预测的潜在生物标志物。