SNP genotype data from induced Pluripotent Stem cells from healthy control donors

We have generated human induced Pluripotent Stem cells (hiPSc) using Retroviral or Sendai virus-mediated delivery of reprogramming factors. hiPSc lines have been screened using SNP array to assess chromosomal stability (alongside the fibroblast lines from which they derived), and validation of the pluripotency of the hiPSc lines is provided by Pluritest assessment of transcriptome datasets, prior to differentiation and downstream assays. MAPT genetic variation and neuronal maturity alter isoform expression affecting axonal transport in iPSC-derived dopamine neurons. Joel E Beevers, Mang-Ching Lai, Heather A Booth, Federico Zambon, Laura Parkkinen, Jane Vowles, Sally A Cowley, Richard Wade-Martins, Tara M Caffrey. Stem Cell Reports 2017 human iPSc lines were derived from human dermal fibroblasts from 3 control donors (newly-derived lines are reported here; for lines that have been previously described and for fibroblasts from which lines have been derived, refer to current manuscript for relevant references). SNP and gene expression datasets were generated.

本研究采用逆转录病毒或仙台病毒介导的重编程因子递送方案，生成人诱导多能干细胞（human induced pluripotent stem cells，hiPSc）。研究团队通过单核苷酸多态性芯片（SNP array）对hiPSc细胞系及其来源的成纤维细胞系开展染色体稳定性筛查，并在细胞分化与下游实验开展前，借助转录组数据集的多能性检测（Pluritest）分析完成hiPSc细胞系的多能性验证。MAPT基因变异与神经元成熟度可调控异构体表达，进而影响诱导多能干细胞来源多巴胺神经元的轴突运输功能。本研究相关成果由Joel E Beevers、Mang-Ching Lai、Heather A Booth、Federico Zambon、Laura Parkkinen、Jane Vowles、Sally A Cowley、Richard Wade-Martins、Tara M Caffrey完成，发表于《Stem Cell Reports》2017年刊。本次报道的人诱导多能干细胞系均来源于3名健康对照供体的皮肤成纤维细胞（本文首次报道本次新建的细胞系；对于此前已报道的细胞系及其来源的成纤维细胞，相关参考文献详见本文手稿）。本研究已生成单核苷酸多态性芯片数据集与基因表达数据集。