Gene expression profile in adipocytes and stromal-vascular cells isolated from interscapular adipose tissue of Syrian hamsters. Mesocricetus auratus

In Syrian hamsters, interscapular adipose tissue is composed primarily of white adipocytes at birth, which is converted to BAT through the proliferation and differentiation of brown adipocyte progenitors and the simultaneous disappearance of white adipocytes. We conducted RNA-seq analysis to profile the gene expressions in stromal-vascular(SV) cells and adipocytes, Interscapular adipose tissue of a 10-day-old hamster was digested with collagenase and fractioned into SV cells and white adipocytes, and used for RNA extraction.

在叙利亚仓鼠中，新生个体的肩胛间脂肪组织主要由白色脂肪细胞构成，该组织可通过棕色脂肪细胞祖细胞的增殖与分化，以及白色脂肪细胞的同步消亡，转化为棕色脂肪组织（BAT）。本研究开展RNA测序（RNA-seq）分析以表征基质血管组分（SV）细胞与脂肪细胞的基因表达谱：取10日龄叙利亚仓鼠的肩胛间脂肪组织，经胶原酶消化后分离得到SV细胞与白色脂肪细胞，并将其用于RNA提取。