3D multiplane dSTORM imaging of whole nuclear lamina

Raw data of single molecule photoswitching for 3D multiplane dSTORM of whole COS-7 nuclear lamina. Data from the first z plane, taken at time = 0 h, and the last z plane, taken at time = 1 h, are shown. Movies were captured at 50Hz under high laser power and in the presence of a reducing switching buffer.Also included is the complete SM coordinate list text file to reconstruct the 3D image of the whole COS-7 nuclear lamina.用于完整COS-7细胞核纤层三维多平面直接随机光学重构显微镜（dSTORM）成像的单分子光切换原始数据集。本次展示了采集于0小时时程的首个z轴平面，以及采集于1小时时程的末位z轴平面的对应数据。所有成像视频均以50Hz帧率采集，实验条件为高激光功率及还原型切换缓冲液。本数据集同时包含用于重构完整COS-7细胞核纤层三维图像的全套单分子（Single Molecule, SM）坐标列表文本文件。