mRNA expression of Icotinib-resistant PC-9 cell lines

Icotinib-resistant cell lines were generated by growing icotinib-sensitive EGFR-mutant NSCLC cell lines (PC9) in increasing concentrations of icotinib to a final concentration of 10 uM up to 12 months.通过将埃克替尼（Icotinib）敏感的表皮生长因子受体（EGFR）突变非小细胞肺癌（NSCLC）细胞系PC9置于浓度逐步升高的埃克替尼培养基中持续培养长达12个月，直至埃克替尼最终浓度达到10 μM，成功构建了埃克替尼耐药细胞系。