Whole Genome Bisulfite Sequencing (WGBS) of cKIT+ sorted cells from 57-137 day old fetal testes and ovaries.

We performed WGBS analyses on 6 human fetal samples at 53-137 days of development, 4 female and 2 male. We show that methylation reprogramming in the human germline is global yet incomplete with exons, 3’UTRs and human-specific transposons remaining methylated. Whole Genome Bisulfite-Seq of cKIT+ cells analyzed from 4 biological samples for fetal ovaries from 57-113 days of development and 2 samples for fetal testes at 59 and 137 days of development.

我们对6例发育时长为53~137天的人类胎儿样本开展了全基因组亚硫酸氢盐测序（Whole Genome Bisulfite Sequencing, WGBS）分析，其中雌性4例、雄性2例。本研究证实，人类生殖系的甲基化重编程具有全局性但并不完全，外显子、3'非翻译区（3' Untranslated Regions, 3'UTRs）以及人类特异性转座子仍保持甲基化状态。本研究同时对cKIT阳性（cKIT-positive）细胞开展全基因组亚硫酸氢盐测序分析，所用样本包括4例发育时长为57~113天的胎儿卵巢生物学重复样本，以及2例分别取自59天和137天发育阶段的胎儿睾丸样本。