RNA-seq analysis of Arabidopsis treated with abscisic acid (ABA) and its derivatives

Transcriptome of chemical-treated plants were investigated for the evaluation of ABA derivatives. Abscisic acid and its derivatives up-regulated mainly abiotic stress responsive genes. Overall design: mRNA profiles of 7-day old wild type Arabidopsis seedlings treated with DMSO, ABA, and two ABA derivatives, in triplicate, using Illumina HiSeq.

本研究通过分析化学试剂处理后的植物转录组（transcriptome），对脱落酸（Abscisic acid，ABA）衍生物进行评估。脱落酸及其衍生物主要上调非生物胁迫响应基因的表达。整体实验设计：采用Illumina HiSeq测序平台，对经二甲基亚砜（DMSO）、脱落酸以及两种脱落酸衍生物处理的7日龄野生型拟南芥幼苗开展mRNA表达谱分析，每组设置三次生物学重复。