Time-course expression data from HEK293∆RAF1:ER cells stimulated with 4OHT, U0126, CYHX, ActD, EGF, FGF, or IGF. Homo sapiens

We integrate experimental data and mathematical modelling to unveil how ERK signal duration is relayed to mRNA dynamics. Overall design: HEK293 cells were transfected with an inducible form of RAF (∆RAF1:ER) and induced with tamoxifen (4OHT) or stimulated with different growth factors (EGF, FGF, iGF). Effects of RAF signalling were perturbed subsequently or in parallel with MEK inhibtor U0126, translation inhibtor cycloheximide (CYHX) and actinomycin D (ActD).

本研究整合实验数据与数学建模手段，揭示细胞外调节蛋白激酶（ERK）信号时长向mRNA动态变化的传递机制。
实验设计概况：将HEK293细胞转染诱导型RAF（∆RAF1:ER），随后以他莫昔芬（4OHT）诱导处理，或采用表皮生长因子（EGF）、成纤维细胞生长因子（FGF）、诱导型生长因子（iGF）等不同生长因子刺激细胞；后续或同步施加丝裂原活化蛋白激酶激酶（MEK）抑制剂U0126、翻译抑制剂环己酰亚胺（CYHX）与放线菌素D（ActD），以扰动RAF信号通路的效应。