Expression data from embryonic day 15.5 atrioventricular canal regions were isolated from Scx-/- and Scx+/+ mice.. Mus musculus

Purpose: Our lab has previously shown that Scleraxis (Scx) is require for proper valve development in vivo. In order to fully explore gene networks regulated by Scx during the vital stages of valve remodeling , high throughput RNA-squencing was performed. Results:There were a total of 18,810 genes were detected. A total of 864 genes were differentially expressed Scx null AVC regions: 645 being upregulated and 217 downregulated. Overall design: In this data set, we include expression data from atrioventricular canal (AVC) regions from Scx null and wild-type littermate controls at embryonic day 15.5. A total of 6 samples were analyzed; 3 valve regions from E15.5 Scx-/- mice, and 3 from E15.5 Scx+/+ wild-type littermate controls. Differential expression read counts are ranked based on p-value (<0.05).

### 研究目的
本课题组前期研究证实，硬化蛋白（Scleraxis, Scx）是体内心脏瓣膜正常发育所必需的。为全面解析瓣膜重塑关键阶段中Scx所调控的基因调控网络，本研究开展了高通量RNA测序（RNA-sequencing）实验。
### 研究结果
本次检测共鉴定到18810个基因。在Scx敲除房室管（atrioventricular canal, AVC）区域中，共发现864个差异表达基因，其中645个基因表达上调，217个基因表达下调。
### 实验设计概述
本数据集包含胚胎发育第15.5天（E15.5）Scx敲除小鼠及其野生型同窝对照的房室管区域转录组表达数据。本次分析共纳入6个样本：3个来自E15.5 Scx-/-小鼠的瓣膜区域，另外3个来自同窝野生型（Scx+/+）小鼠对照。差异表达读段计数基于P值（<0.05）进行排序。