Protease activities induced in the apoplast of the resistant tomato cultivar Hawaii 7996 in response to Ralstonia solanacearum infection

In the work presented here we aimed to identify the active proteases secreted in the apoplast of the resistant tomato Hawaii 7996 when challenged with R. solanacearum and compared it with that of the susceptible cultivar Marmande. Using ABPP we characterized a variety-specific induction of papain-like cysteine proteases and serine hydrolases.. Altogether this work denotes the importance of novel proteomic approaches –such as ABPP– to provide new insights on old yet elusive questions regarding the molecular basis of resistance to R. solanacearum.

本研究旨在鉴定接种青枯劳尔氏菌（R. solanacearum）后，抗病番茄品种夏威夷7996（Hawaii 7996）质外体（apoplast）中分泌的活性蛋白酶，并将其与感病品种马尔曼德（Marmande）的对应结果进行对比。本研究采用活性探针蛋白质组学（ABPP）技术，解析了木瓜蛋白酶样半胱氨酸蛋白酶与丝氨酸水解酶的品种特异性诱导特征。综上，本研究彰显了新型蛋白质组学技术（如ABPP）的重要价值，可为解析青枯病抗病分子机制这一长期存在却仍难以阐明的科学问题提供全新视角。