Soybean Compact Mutant

Genome resequencing for mutation discovery in soybean short internode mutant. Whole-genome sequencing of the mutant revealed an 8.7kb deletion in the promoter of the Glyma.17g145200 gene, which encodes a member of the class III gibberellin 2-oxidases. The mutation has a dominant effect, likely via increased expression of the GA 2-oxidase transcript observed in green tissue as a result of the deletion in the promoter of Glyma.17g145200. Levels of GA precursors are altered in the Compact mutant, supporting a role in gibberellin metabolism, and the mutant phenotype can be rescued with exogenous GA3.

本数据集围绕大豆节间短突变体的突变发掘开展基因组重测序研究。对该突变体进行全基因组测序后，检测到其Glyma.17g145200基因的启动子区域存在一段8.7kb的缺失；该基因编码Ⅲ类赤霉素2-氧化酶（class III gibberellin 2-oxidases）家族成员。该突变呈现显性遗传效应，其潜在机制为：Glyma.17g145200基因启动子的缺失导致绿色组织中赤霉素2-氧化酶转录本的表达量显著升高。该紧凑突变体体内的赤霉素前体水平发生异常变化，证实该突变参与赤霉素代谢过程；且通过外源施加赤霉素3（GA3）可恢复该突变体的异常表型。