Transcriptome profile of gene expression parttern in TYLCV-infected whiteflies over different viral acquisition access periods.

Tomato yellow leaf curl virus (TYLCV) transmitted by Bemisia tabaci is a great threat to agricultural production. To detect the global gene changes in TYLCV-infected whiteflies, in this study, comparative transcriptomic analyses were conducted by RNA-seq on whiteflies following 0, 2, 6, 24 and 48 h viral acquisition access period. Totally, there were 244 up-regulated and 511 down-regulated, 302 up-regulated and 285 down-regulated, 392 up-regulated and 748 down-regulated, 393 up-regulated and 954 down-regulated DEGs in the comparison of 0 h vs. 2 h, 0 h vs. 6 h, 0 h vs. 12 h and 0 h vs. 48 h , respectively. Compared with un-infected whiteflies, similar regulations of metabolism and signal transductions were found in viruliferous whiteflies. Meanwhile, time-associated gene cluster analysis revealed 16 temporally up-regulated transporter genes and 10 down-regulated genes associated with IL-17 signaling pathway in viruliferous whiteflies. These data boost our comprehensions on whitefly-TYLCV interactions.

番茄黄化曲叶病毒（Tomato yellow leaf curl virus, TYLCV）由烟粉虱（Bemisia tabaci）传播，对农业生产构成严重威胁。为探究感染TYLCV的烟粉虱的全局基因表达变化，本研究针对获毒时长分别为0、2、6、24和48小时的烟粉虱，通过RNA测序（RNA-seq）开展了比较转录组分析。在0小时与2小时、0小时与6小时、0小时与12小时以及0小时与48小时的组间比较中，分别鉴定得到244个上调、511个下调；302个上调、285个下调；392个上调、748个下调；以及393个上调、954个下调的差异表达基因（differentially expressed genes, DEGs）。与未感染病毒的烟粉虱相比，带毒烟粉虱在代谢与信号转导通路中呈现相似的表达调控模式。同时，时间关联基因簇分析显示，带毒烟粉虱中存在16个时序上调的转运蛋白基因，以及10个与白细胞介素-17（Interleukin-17, IL-17）信号通路相关的下调基因。本研究数据将有助于深化我们对烟粉虱与TYLCV互作机制的理解。