PHYTOCHEMICAL STUDY AND EVALUATION OF THE ANTIOXIDANT ACTIVITIES OF LEAVES FROM VERNONIAAMYGDALINA

The aim of this study is to highlight the phytochemical composition and antioxidant potential of the ethereal extract of the leaves of Vernoniaamygdalina, a plant used in traditional medicine in Côte dIvoire.Phytochemical studies using TLC and LC-MS revealed, in addition to coumarins, flavonoids and phenolic acids. Determination of the antioxidant potential by TLC and spectrophotometry using the DPPH radical showed the antioxidant power of Vernoniaamygdalina leaves with a CR50 of 0.01995 ± 0.2x10-5 % mg/mL.The many pharmacological properties of V. amygdalina would therefore depend on the coexistence of these phytocompounds.

本研究旨在阐明科特迪瓦传统药用植物扁桃斑鸠菊（Vernonia amygdalina）叶片乙醚提取物的植物化学成分组成与抗氧化活性。采用薄层色谱（Thin Layer Chromatography, TLC）与液相色谱-质谱联用（Liquid Chromatography-Mass Spectrometry, LC-MS）开展的植物化学研究结果显示，该提取物中含有香豆素类、黄酮类及酚酸类等成分。采用薄层色谱、分光光度法结合1,1-二苯基-2-三硝基苯肼（DPPH）自由基法测定其抗氧化活性，结果表明扁桃斑鸠菊叶片提取物具备抗氧化能力，其半数抑制浓度（CR50）为0.01995 ± 0.2×10^-5 % mg/mL。因此，扁桃斑鸠菊（V. amygdalina）的多种药理活性均有赖于这些植物活性成分的协同共存。