CSC⁺ and CSC⁻ tumor cells display similar tumor-forming capacity upon co-transplantation.

(A and B) DsRed-labeled CSC+ and EGFP-labeled CSC− cells originating from the same tumor cell lines were mixed to their original ratio and co-transplanted into sublethally irradiated NOD-SCID mice. Photographs represent fluorescent images (A) and hematoxylin and eosin staining (B) of serial sections of the xenografts derived from KG-1, MCF-7, SHG44, Caco-2 and A375. Scale bar = 100 μm. (C) Flow cytometric contour plots show the percentage of DsRed and EGFP cells in the xenografts. (D) Histograms show the ratio of CSC+ to CSC− (DsRed:EGFP) cells in injections and the ratio of CSC+-derived to CSC−-derived (DsRed:EGFP) cells in xenografts. Data represent mean ± SEM from 3 independent experiments.(A与B) 源自同一肿瘤细胞系的、经DsRed标记的肿瘤干细胞（Cancer Stem Cell, CSC）阳性（CSC+）细胞与经增强绿色荧光蛋白（EGFP, Enhanced Green Fluorescent Protein）标记的肿瘤干细胞阴性（CSC−）细胞，按照原始比例混合后，共同移植至经亚致死剂量辐照的NOD-SCID小鼠体内。所展示的图像为来自KG-1、MCF-7、SHG44、Caco-2及A375细胞系的异种移植瘤连续切片的荧光图像（A）与苏木精-伊红染色图像（B），比例尺为100 μm。(C) 流式细胞术轮廓图展示了异种移植瘤中DsRed阳性细胞与EGFP阳性细胞的占比。(D) 柱状图分别呈现了接种体系中CSC+与CSC−细胞的比例（DsRed:EGFP），以及异种移植瘤中CSC+来源细胞与CSC−来源细胞的比例（DsRed:EGFP）。所有数据均为3次独立实验的均值±标准误（SEM, Standard Error of the Mean）。