Transcriptomic analysis of evolved strains.

We develop a CRISPR-Cas assisted random mutation (CARM) technology for whole genome mutagenesis. As a proof-of-principle, CARM was applied to evolve the capacity of Saccharomyces cerevisiae to produce ß-carotene. Thees files are transcriptomic analysis of finally evolutionary strain C7-143, processed evolutionary strain C3-06 and C5-63, and the original strains C0 and BY47419. Overall design: Transcriptomic analysis of evolved strains.

我们开发了一种用于全基因组诱变的CRISPR-Cas辅助随机突变（CRISPR-Cas assisted random mutation, CARM）技术。作为原理验证应用，我们将CARM技术应用于改造酿酒酵母（Saccharomyces cerevisiae）的β-胡萝卜素合成能力。本数据集包含最终进化菌株C7-143、进化处理菌株C3-06与C5-63，以及原始菌株C0和BY47419的转录组分析数据。整体实验设计：进化菌株的转录组分析。