Enrichment of soil-associated communities under denitrifying conditions

In order to investigate the genotypes and ecological interactions present in denitrifying communities along an environmental gradient in pH, soils were sampled from multiple locations, homogenized, and used to inoculate defined medium with nitrate supplied as the sole electron acceptor. Cultures were incubated under anaerobic conditions and then serially propagated for 12 cycles of 72 h each. DNA was extracted from endpoint cultures and sequenced using an Illumina NextSeq 550 (2x150 paired end reads). The raw sequencing reads from this experiment are deposited on the SRA.

为探究沿pH环境梯度分布的反硝化群落中的基因型与生态互作，研究人员从多个地点采集土壤样品，经均质化处理后，以硝酸盐作为唯一电子受体接种至限定培养基中。培养物于厌氧条件下孵育，随后连续传代12个周期，每个周期时长为72小时。研究人员从终点培养物中提取DNA，使用Illumina NextSeq 550测序平台进行2×150 bp配对末端读段（paired end reads）测序。本实验产生的原始测序读段已提交至序列读段存档库（Sequence Read Archive, SRA）。