Control of Reproductive Floral Organ Identity Specification in Arabidopsis by the C Function Regulator AGAMOUS (expression). Arabidopsis thaliana

Characterization of the activities of the transcription factor that AG encodes throughout flower development using perturbation assays in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. The series contains static perturbation experiments (null allele ag-1), and amiRNA knockdown experiments. Overall design: Two conditions (i.e. perturbation experiments of ag-1 homozygous in the FIS vs ag-1 heterozygous in the FIS across three developmental stages. amiRNA knockdown experiments induced amiRNA expression vs. uninduced.

本数据集通过结合可实现花发育阶段特异性分析的花诱导系统（floral induction system, FIS）与扰动实验，对AG编码的转录因子在整个花发育过程中的活性进行表征。该数据集系列包含两类实验：采用ag-1纯合无效等位基因的静态扰动实验，以及人工微小RNA (amiRNA) 敲低实验。实验整体设计包含两类实验条件：其一为花诱导系统中ag-1纯合突变体与ag-1杂合突变体在三个发育阶段的扰动实验；其二为amiRNA诱导表达与未诱导表达的对照实验。