SPA regulation of high ambient temperature induced gene expressions in Arabidopsis thaliana.

The mRNA expression profiles of WT and the quadruple spa mutant spaQ were analyzed in 24 hour 22degC and 28degC treated condition. The gene expression changes was analyzed and compared within 22degC and 28degC. Overall design: Total twelve 24h 22degC and 28degC treated Arabidopsis samples were used. These include three replicates of following genotypes; Col-0, spaQ. Therefore, three replicates of following genotypes and conditions; Col-0 (22degC), spaQ (22degC0, Col-0 (28degC), spaQ (28degC).

本研究分析了野生型（Wild Type，WT）与四重spa突变体spaQ（quadruple spa mutant spaQ）在22℃、28℃条件下处理24小时后的mRNA表达谱（mRNA expression profiles）。针对22℃与28℃两种处理条件下的基因表达变化，本研究开展了分析与比较。整体实验设计如下：共使用12份经22℃、28℃处理24小时的拟南芥（Arabidopsis）样本，包含两种基因型的3次生物学重复：Col-0与spaQ。具体而言，各处理组合均设置3次重复，分别为Col-0（22℃）、spaQ（22℃）、Col-0（28℃）及spaQ（28℃）。