CUSP9-9095

Exhaustive in vitro evaluation of the 9-drug cocktail CUSP9 for treatment of glioblastoma using COMBImageDL. Label-free live-cell imaging data for the second replicate 384-well plate (barcode 9095) containing all single drugs (at one fixed concentration each) and all plausible drug combinations up to order 4 from the CUSP9 protocol as add-on to standard-of-care Temozolomide, using the human glioblastoma cell line M059K. 22 grayscale images/frames of size 800x992 were acquired per experimental well, one every 4h for 84h, starting without treatment at 0h. The first and second channel of each frame contain the original grayscale pixel values, while the third one contains the binary segmentation map (foreground vs. background) generated with a modified version of the deep convolutional neural network architecture U-Net. M059K_190429_9095.xlsx: Excel file containing the experimental layout designed for plate 9095 and used to produce the corresponding transfer scheme for acoustic liquid dispension.

本数据集针对胶质母细胞瘤（glioblastoma）治疗用9药鸡尾酒疗法CUSP9，借助COMBImageDL开展了全面的体外评价实验。实验采用人胶质母细胞瘤细胞系M059K，以临床标准疗法替莫唑胺（Temozolomide）为基础用药，针对第二次重复实验的384孔板（条形码编号9095）获取无标记活细胞成像数据；该孔板包含CUSP9方案中所有单一药物（各使用固定单一浓度）以及最多4种药物的所有合理联合用药组合。每个实验孔均采集22帧尺寸为800×992的灰度图像，从0h（未给药初始状态）开始，每4h采集1帧，总时长84h。每帧图像的前两个通道存储原始灰度像素值，第三个通道则为经改进版深度卷积神经网络架构U-Net（U-Net）生成的二值分割掩码（用于区分前景与背景）。M059K_190429_9095.xlsx：该Excel文件记录了9095号孔板的实验布局，用于生成声波液体分配的对应传输方案。