IL-9 in psoriasis: human endothelial cells and murine T cells

Atherosclerotic cardiovascular disease (ASCVD) is a leading cause of death in patients wiht systemic autoimmune and inflammatory diseases. The inflammatory cytokine interleukin 9 (IL-9) has been linked to ASCVD atherogenesis, raising fundamental questions about the populations in which and the mechanisms by which IL-9 drives ASCVD, leading us to investigate IL-9 in psoriasis. This incuded mRNA sequencing of IL-9+ and IL-9- circulating CD4+ T cells from INFER (IL-9 reporter) mice treated with imiquimod to induce psoriatic inflammation, as well as vehicle vs. IL-9-treated primary human aortic endothelial cells Overall design: Experiment 1: mRNA sequencing of IL-9+ and IL-9- circulating CD4+ T cells from INFER (IL-9 reporter) mice treated with imiquimod for 6 weeks to induce psoriatic inflammation Experiment 2: mRNA sequencing of primary human aortic endothelial cells (HAoEC) exposed to IL-9 vs. vehicle for 16 hours

动脉粥样硬化性心血管疾病（Atherosclerotic cardiovascular disease, ASCVD）是系统性自身免疫与炎症性疾病患者的首要致死病因。炎性细胞因子白细胞介素9（interleukin 9, IL-9）已被证实与ASCVD的动脉粥样硬化发生发展相关，由此引出了关于IL-9驱动ASCVD的易感人群及具体作用机制的基础科学问题，为此我们在银屑病模型中开展了IL-9相关研究。本研究的测序实验包含两部分：一是对经咪喹莫特（imiquimod）诱导银屑病炎症6周的INFER（IL-9报告基因）小鼠的循环IL-9阳性与IL-9阴性CD4+ T细胞进行mRNA测序；二是对经IL-9与对照溶剂处理16小时的原代人主动脉内皮细胞（primary human aortic endothelial cells, HAoEC）进行mRNA测序。整体实验设计如下：实验1：对经咪喹莫特诱导银屑病炎症6周的INFER（IL-9报告基因）小鼠的循环IL-9阳性与IL-9阴性CD4+ T细胞进行mRNA测序；实验2：对经IL-9与对照溶剂处理16小时的原代人主动脉内皮细胞进行mRNA测序。