Analysis of transgenerational effects on DNA copy number aberrations in male mice exposed to continuous 20mGy/day gamma-rays for 400 days (Primary screening for 0mGyP familly).

Transgenerational effects of continuous low dose-rate (LDR) gamma-ray irradiation have not been well studied. C57BL/6JNrs male mice were maintained in non-irradiated room for 400 days from 8 weeks of age. This study investigated the De novo mutation by comparing the parents and children. This is primary screening. This study was performed under contract with the Aomori Prefectural Government, Japan. Two-condition experiment, C57 BL/6JNrs tail tissue non-irradiated male (0mGyPM), female (0mGyPF) and their progenies (0mGyP1 to 0mGyP7) vs. reference male mouse.

持续低剂量率（low dose-rate, LDR）γ射线辐照的跨代效应尚未得到充分研究。本研究将8周龄的C57BL/6JNrs雄性小鼠置于非辐照饲养环境中饲养400天，通过比较亲代与子代样本探究新发突变（de novo mutation）情况，本次实验属于初步筛选阶段。本研究根据与日本青森县政府签订的合同开展。本实验为双组对照实验：以未接受辐照的C57BL/6JNrs雄性小鼠尾部组织（0mGyPM）、雌性小鼠尾部组织（0mGyPF）及其子代尾部组织（0mGyP1至0mGyP7）为受试样本，与参考雄性小鼠进行对照。