U2OS Cell Cycle Transition Curve-- NCS 300 ng/mL

U2OS cells expressing the PCNA-mCherry reporter were induced with 300 ng/mL NCS at 20.4 hours into the movie and then imaged for another 48 hours. Time-lapse images were taken every 20 minutes.将表达PCNA-mCherry报告基因（PCNA-mCherry reporter）的U2OS细胞，在延时成像启动后20.4小时时，以300 ng/mL的NCS诱导处理，随后进行为期48小时的成像采集；该延时成像的图像采集间隔为每20分钟一次。