Transcriptomic analysis of whole mouse tibia samples. Transcriptomic analysis of whole mouse tibia samples

The combined effects of aging and a dietary intervention of reduced energy intake, caloric restriction, were examined by conducting a bulk transcriptomic analysis of mouse whole tibia bone samples. Tissue samples were collected from mice at two different age groups, comparing old and young animals, with caloric restriction in comparison to full food access. Overall design: Male C57Bl6J mice in two age groups, young mice aged 10 weeks and older mice, aged between 15-18 months, underwent caloric restriction for 8 weeks and were compared to age-matched control mice with free food access. Mice fed with the control diet received the NIH-31 rodent open formula diet, while caloric restriction mice were provided with a modified NIH-31/NIA diet, which is fortified with micronutrients. In the first week of caloric restriction, mice received a 10% restriction, followed by 20% restriction in the second week, and a restriction of 40% from the third week onward until the end of the experiment.

本研究通过对小鼠全胫骨骨样本开展批量转录组分析（bulk transcriptomic analysis），探究了衰老与能量摄入减少的饮食干预——热量限制（caloric restriction）的联合效应。实验采集了两个不同年龄组小鼠的组织样本，比较老年与年轻个体，并设置热量限制组与自由进食组进行对照。实验整体设计：选取雄性C57Bl6J小鼠，分为两个年龄组——10周龄的年轻小鼠，以及15至18月龄的老年小鼠，所有小鼠均接受为期8周的干预，其中热量限制组与同龄的自由进食对照组小鼠进行比较。对照组小鼠喂食NIH-31啮齿类开放式配方饲料（NIH-31 rodent open formula diet），而热量限制组小鼠则喂食添加了微量营养素的改良型NIH-31/NIA饲料。在热量限制干预的第一周，小鼠实施10%的进食限制，第二周实施20%的进食限制，自第三周起直至实验结束，维持40%的进食限制。