Upstream regulator of genomic imprinting in rice is a small RNA-associated chromatin remodeler [targeted bisulfite PCR]. Upstream regulator of genomic imprinting in rice is a small RNA-associated chromatin remodeler [targeted bisulfite PCR]

Knock down of CLSY3 in rice caused endosperm development defects. We investigated the molecular effects of loss of CLSY3 in rice endosperm by profiling sRNA, targeted bisulfite PCR and mRNA transcriptomes .These analyses revealed the roles of CLSY3 in Endosperm Overall design: We have performed targeted bisulfite PCR

水稻中CLSY3基因敲低可导致胚乳发育缺陷。本研究通过对小RNA（sRNA）、靶向亚硫酸氢盐PCR（targeted bisulfite PCR）以及mRNA转录组开展测序分析，探究了水稻胚乳内CLSY3功能缺失所产生的分子效应。上述分析揭示了CLSY3在胚乳中的功能作用。整体实验设计：本研究已完成靶向亚硫酸氢盐PCR实验。