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Plant cell cultures constitute a potentially efficient and sustainable tool for the production of high added-value bioactive compounds. However, due to the inherent restrictions in the expression of secondary metabolism, to date the yields obtained have generally been low. Plant cell culture elicitation can boost production, sometimes leading to dramatic improvements in yield, as well as providing insight into the target biosynthetic pathways and the regulation of the genes involved. Among the secondary compounds successfully being produced in biotechnological platforms are taxanes and trans-resveratrol (t-R). In the current study, perfluorodecalins (PFDs) and hexenol (Hex) were tested for the first time with Taxus media and Vitis vinifera cell cultures to explore their effect on plant cell growth and secondary metabolite production, either alone or combined with other elicitors already established as highly effective, such as methyl jasmonate (MeJa), coronatine (Coro) or randomly methylated β-cyclodextrins (β-CDs). The total taxane content at the peak of production in T. media cell cultures treated with PFDs together with Coro plus β-CDs was 3.3-fold higher than in the control, whereas the t-R production in MeJa and β-CD-treated V. vinifera cell cultures increased 552.6-fold compared to the extremely low-yielding control. Hex was ineffective as an elicitor in V. vinifera cell cultures, and in T. media cell suspensions it blocked the taxol production but induced a clear enhancement of baccatin III. Regarding biosynthetic gene expression, a strong positive relationship was observed between the transcript level of targeted genes and taxol production in the T. media cell cultures, but not with t-R production in the elicited V. vinifera cell cultures.

植物细胞培养（Plant cell cultures）是生产高附加值生物活性化合物的一种极具潜力且可持续的手段。然而，由于次生代谢（secondary metabolism）表达存在固有局限，目前所获产物产量普遍偏低。对植物细胞培养进行激发诱导，不仅可提升产物产量，有时还能实现产率的显著提升，同时有助于解析目标生物合成途径及相关基因的调控机制。目前已在生物技术平台中成功量产的次生代谢产物包括紫杉烷类（taxanes）与反式白藜芦醇（trans-resveratrol, t-R）。在本研究中，研究人员首次将全氟萘烷（perfluorodecalins, PFDs）与己烯醇（hexenol, Hex）应用于曼地亚红豆杉（Taxus media）及葡萄（Vitis vinifera）细胞培养体系，以探究其单独或与已被证实高效的其他激发子（elicitors）——如茉莉酸甲酯（methyl jasmonate, MeJa）、冠菌素（coronatine, Coro）或随机甲基化β-环糊精（randomly methylated β-cyclodextrins, β-CDs）——联合使用时，对植物细胞生长及次生代谢产物（secondary metabolite）合成的影响。经PFDs与Coro及β-CDs联合处理的曼地亚红豆杉细胞培养体系中，产率峰值时的总紫杉烷含量较对照组提升3.3倍；而经MeJa与β-CD处理的葡萄细胞培养体系中，t-R产量较产量极低的对照组提升552.6倍。己烯醇（Hex）在葡萄细胞培养体系中作为激发子并无效果，而在曼地亚红豆杉细胞悬浮培养体系中，其会抑制紫杉醇（taxol）的合成，但可显著提升巴卡丁III（baccatin III）的产量。在生物合成基因表达层面，曼地亚红豆杉细胞培养体系中，目标基因的转录水平与紫杉醇产量呈显著正相关；而在经激发诱导处理的葡萄细胞培养体系中，该相关性并未体现在t-R产量与对应基因转录水平之间。