Deep transcriptomics analysis reveals the development of postoperative ileus and the therapeutic effect of electroacupuncture. Deep transcriptomics analysis reveals the development of postoperative ileus and the therapeutic effect of electroacupuncture

Postoperative ileus (POI) often occurs and prolongs hospital stay, which costs expensive. Electroacupuncture (EA), as an appropriate non-pharmacotherapy supported by emerging clinical trials, the mechanism needs to be clarified. In this study, the intestinal manipulation (IM) method was used to establish the POI model, and EA was used to treat it. Sham surgery (SS), simple electrical stimulation (ES), and sham electroacupuncture (SEA) were introduced as control. Gastrointestinal transit and colonic transport were employed to evaluate interventions’ effects. RNA sequencing was then carried out using the intestine tissue of mice. Overall design: Intestine mRNA profiles of C57BL/6 mice.

术后肠梗阻（Postoperative Ileus, POI）较为常见，可延长患者住院时间并造成高额医疗开支。电针（Electroacupuncture, EA）作为一种经新兴临床试验验证的非药物治疗手段，其作用机制仍有待阐明。本研究采用肠牵拉（Intestinal Manipulation, IM）法构建POI小鼠模型，并施加电针干预，同时设置假手术（Sham Surgery, SS）、单纯电刺激（Simple Electrical Stimulation, ES）与假电针（Sham Electroacupuncture, SEA）作为对照。通过检测胃肠道转运与结肠传输功能以评估各干预措施的效果，随后提取小鼠肠组织进行RNA测序。整体实验设计：C57BL/6小鼠的肠道mRNA表达谱。