A conserved RNA-binding protein Celf1 controls cell differentiation in vertebrate eye development

Celf1 germline or conditional deletion mouse mutants exhibit fully penetrant lens defects including cataract. To gain insight into gene expression changes underlying these lens defects, microarray comparison was performed for lenses obtained from wild-type and Celf1 conditional deletion mutant mice. At ±2.0 fold-change cut-off (p<0.05), 102 genes were identified to be differentially expressed in Celf1 conditional mutant lenses Microarray comparison of lenses from mixed background (C57BL/6J, FVB) wild-type and mutant (Celf1cKO/lacZKI; Pax6GFPCre) mice was performed

Celf1生殖系或条件性敲除小鼠突变体可呈现完全外显的晶状体缺陷，涵盖白内障表型。为深入解析此类晶状体缺陷背后的基因表达变化，研究人员对野生型与Celf1条件性敲除突变小鼠的晶状体开展了微阵列芯片（microarray）比较分析。在±2.0倍差异阈值（p<0.05）的筛选标准下，共鉴定出102个在Celf1条件性突变小鼠晶状体中存在差异表达的基因。本次分析针对混合遗传背景（C57BL/6J、FVB）的野生型与突变型（Celf1cKO/lacZKI; Pax6GFPCre）小鼠的晶状体完成了微阵列芯片比较分析。