Multi-colour SMLM images of Escherichia coli cells with varying RNaseE localization and expression (LB, exponential phase)

This dataset is part of the publication "The nucleoid of rapidly growing Escherichia coli localizes close to the inner membrane and is organized by transcription, translation and cell geometry". It contains the SMLM images of RNase E experiments, in which the localization and abundance of RNase E was altered. It also includes the regions of interest used for the analyses. Cultures were then chemically fixed, permeabilised and imaged for the nucleoid (JF646-Hoechst) and membranes (Nile Red) using PAINT. Strains MBS106 (NCM3416, rne-frt) (Reference), MBS157 (NCM3416, rneΔMTS-frt) (Reference) and SLP60 (pKti4/Δrne-frt) were kind gifts of Agamemnon J. Carpousis. More information can be found in the publication.本数据集来自发表论文《快速生长大肠杆菌（Escherichia coli）的类核定位于内膜附近，并通过转录、翻译及细胞几何结构实现有序组织》。 该数据集包含核糖核酸酶E（RNase E）相关实验的单分子定位显微镜（SMLM）图像，实验中对RNase E的定位与丰度进行了调控；同时还包含了分析所用的感兴趣区域（ROI）。 随后对培养物实施化学固定与透化处理，并采用PAINT成像技术分别对类核（JF646-Hoechst）与细胞膜（尼罗红，Nile Red）进行成像。菌株MBS106（NCM3416, rne-frt）（对照）、MBS157（NCM3416, rneΔMTS-frt）（对照）与SLP60（pKti4/Δrne-frt）由Agamemnon J. Carpousis惠赠。 更多详细信息可参阅该发表论文。