Differential effects of a glucagon-like peptide 1 receptor agonist in NAFLD and in response to hepatectomy

The genomic analysis of liver from mice fed with standard or MCD diet and treated with FC-GLP-1 during two weeks before 70%partial hepatectomy (PH) and after 2 weeks PH resulted in a set of genes regulated by diet and other set regulated differentially by treatment in MCD treated animals. These genes are apparently responsible for the reversion and prevention of NAS and improvement in hepatic regeneration induced by drug treatment All microarray analyses were performed with RNA samples obtained from four independent liver from animals with different diet and drug treatmens.

本研究对喂食标准饲料或蛋氨酸胆碱缺乏（Methionine-Choline Deficient, MCD）饲料、并在70%部分肝切除术（Partial Hepatectomy, PH）前两周及术后两周接受FC-GLP-1处理的小鼠肝脏开展基因组分析，最终鉴定出两组基因：一组受饮食调控，另一组在MCD饲料喂养的小鼠中经药物处理后存在差异表达。这些基因显然介导了药物处理诱导的非酒精性脂肪性肝炎（Non-Alcoholic Steatohepatitis, NAS）的逆转与预防，同时改善了肝脏再生能力。所有基因芯片（microarray）检测均采用来自不同饮食及药物处理组的4份独立小鼠肝脏组织提取的RNA样本完成。