Long read transcriptome and Differentially expressed genes between sexs of juvenile / adult mud crabs

We hypothsized that SMRT sequencing producing long length reads may provide more conprehensive transcriptome and accurate downstream analysis than next generation sequencing. Here we annotated the unigenes of the long length transcriptome and analysis the DEGs. We found that DEGs were present between sexes during early developmental stages of the mud crab. Several important KEGG pathways were identified to be potentially involved in sex differention
File S1: Unigene annotation in five protein database including non-redundant protein database (NR), Swiss-prot, Clusters of orthologous eucaryotic genes (KOG), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomics (KEGG) databases ;
File S2: Predicted simple sequence repeats （SSRs）in the long read transcriptome of mud crab;
File S3: Differentially expressed genes (DEGs) between juvenile male (CM) and juvenile female (CF) crabs;
File S4: Differentially expressed genes (DEGs) between adult male (AM) and juvenile female (AF) crabs;
File S5: Enriched KEGG pathway analysis of the DEGs between juvenile male (CM) and juvenile female (CF) crabs；
File S6: Enriched KEGG pathway analysis of the DEGs between adult male (AM) and juvenile female (AF) crabs.

我们提出如下假设：相较于下一代测序，可产出长读长序列的单分子实时测序（SMRT, Single-Molecule Real-Time Sequencing）能够为转录组研究提供更为全面的转录组数据与更精准的下游分析结果。本研究对长读长转录组的单基因（unigenes）进行了注释，并开展了差异表达基因（Differentially Expressed Genes, DEGs）分析。我们发现，在泥蟹早期发育阶段，不同性别间存在差异表达基因，且鉴定出若干潜在参与性别分化的重要KEGG通路。
文件S1：涵盖非冗余蛋白质数据库（NR, non-redundant protein database）、Swiss-prot数据库、真核生物直系同源簇（KOG, Clusters of Orthologous Eukaryotic Genes）数据库、基因本体（GO, Gene Ontology）数据库以及京都基因与基因组百科全书（KEGG, Kyoto Encyclopedia of Genes and Genomics）在内的5个蛋白质数据库中的单基因注释信息；
文件S2：包含泥蟹长读长转录组中预测得到的简单序列重复（SSRs, Simple Sequence Repeats）信息；
文件S3：包含幼年雄性泥蟹（CM, juvenile male）与幼年雌性泥蟹（CF, juvenile female）间的差异表达基因数据；
文件S4：包含成年雄性泥蟹（AM, adult male）与幼年雌性泥蟹（AF, juvenile female）间的差异表达基因数据；
文件S5：包含幼年雄性与幼年雌性泥蟹间差异表达基因的富集KEGG通路分析结果；
文件S6：包含成年雄性与幼年雌性泥蟹间差异表达基因的富集KEGG通路分析结果。