Global transcriptomic analysis of a L. monocytogenes agrD mutant

Global expression profiles of L. monocytogenes EGDe and an isogenic agrD deletion mutant were analyzed by custom spotted microarrays (Agilent 4x44k arrays, mono-dye setup) covering all genes of the genome.The file set contains a .zip file with raw data including a detailed descriptions of the design of the array and reports on the analysis procedures. The raw data set comprises a second experiment (transcriptomic analysis), which is irrelevant to the comparison of EGDe WT and the agrD mutant.
For ease of interpretation and analysis there are further files with raw spot intensities of the relevant samples, a sample key list and a list with background-corrected, normalized log2-fold changes in expression in exponential and stationary growth phase for all genes with statistical significance of the change.

本研究采用覆盖全基因组所有基因的定制点样微阵列（custom spotted microarrays，Agilent 4x44k arrays，单染料标记方案），对单核细胞增生李斯特菌EGDe株（Listeria monocytogenes EGDe，缩写L. monocytogenes EGDe）及其同基因agrD缺失突变株的全局表达谱进行了分析。
本次数据集包含一份压缩包文件，内含原始微阵列数据，同时附带微阵列设计的详细说明以及数据分析流程报告。
该原始数据集还包含另一项与EGDe野生型（Wild Type，WT）及agrD突变株的比较分析无关的转录组学实验数据。
为便于解读与分析，数据集还提供了额外文件：包括相关样本的原始点信号强度文件、样本信息对照表，以及涵盖所有基因在指数生长期与稳定生长期的表达量经背景校正、归一化后的log₂倍变化值，并附带该表达变化的统计学显著性检验结果。