Datasets of endodontic microorganisms killed at 265 and 280 nm wavelengths by ultraviolet C light emitting diodes

Treatment of root canals with ultraviolet C (UVC) light-emitting diodes (LED) is a promising adjunct to current root canal disinfection procedures that are essential for preserving the health and the healing of periapical tissues. Here, we hypothesized that UVC LED treatment would kill microorganisms associated with endodontic infections and kill microorganisms in instrumented root canal models. Three different UVC LED units were constructed. One unit emitted 265 nm from a 12 mW LED, one unit emitted 265 nm from a 22.5 mW LED, and one unit emitted 280 nm from an 8 mW LED. Power levels emitted from the three units were measured without and with fiberoptic (FO) filaments using an energy meter (PM100D, Thorlabs, Inc., Newton, NJ USA) with a standard photodiode sensor (S120VC, 200-1100 nm, 50 mW; Thorlabs, Newton, NJ USA) for 30 seconds. The raw data from the energy meter readings for these 3 units is listed in Table 1. This includes the wavelength the meter used to record the data for each unit (Wavelength read at (nm)), the diameters of the hub opening or FO filament emitting the UV light (Diameter of hub opening or FO filament (mm)), the minimum power value recorded from the meter (Minimum value (µW)), the maximum power value recorded from the meter (Maximum value (µW)), the mean value recorded from the meter (Mean value (µW)), the standard deviation of the mean value recorded from the meter (Standard deviation (µW)), the number of meter readings (Number of readings (n)), and the time of recording (Time (sec)). The raw data from the antimicrobial activities of these 3 units on Candida albicans ATCC 64124, Enterococcus faecalis ATCC 29212, Streptococcus sanguinis ATCC 10556, Staphylococcus aureus ATCC 29213, and methicillin-resistant S. aureus (MRSA #7) treated in vitro on discs in laboratory assays is listed in Table 2. This includes the length of time each microorganism was treated (Group (seconds)), the UVC LED unit used to treat each microorganism (UVC LED treatment), the replication number (Replication), the microorganism treated (Microorganism), and the number of viable colony forming units (CFU) of microorganisms surviving UVC LED treatment (Viable CFU). The raw data from the antimicrobial activity of the 265 nm (22.5 mW) unit on E. faecalis ATCC 29212, C. albicans ATCC 64124, MRSA #7, or S. aureus ATCC 29213 in ex vivo models of root canals is listed in Table 3. This includes whether the group was a control or treatment (Group), if there was UVC LED treatment with the 265 nm (22.5 mW) unit (UVC LED treatment), the replication number (Replication), the microorganism treated (Microorganism), and the number of viable CFU of microorganisms surviving 265 nm (22.5 mW) unit treatment (Viable CFU).

采用紫外C（UVC）发光二极管（LED）进行根管辅助治疗，是当前根管消毒流程中极具应用前景的辅助手段，而根管消毒本身对于维持根尖周组织健康、促进其愈合至关重要。本研究提出假设：UVC LED照射可杀灭与牙髓感染相关的微生物，并可杀灭经根管预备后的根管模型内的微生物。本研究构建了三台不同的UVC LED装置：其中一台采用12 mW的LED发射265 nm紫外光，一台采用22.5 mW的LED发射265 nm紫外光，剩余一台采用8 mW的LED发射280 nm紫外光。本研究采用能量计（PM100D，索雷博公司，美国新泽西州牛顿市）搭配标准光电二极管传感器（S120VC，200~1100 nm，50 mW；索雷博公司，美国新泽西州牛顿市），分别在有无光纤（FO）导丝的情况下，对三台装置的输出功率进行了30秒的测量。三台装置的能量计原始测量数据详见表1，该数据集包含各装置的采集波长（Wavelength read at (nm)）、紫外光发射端的接头开孔或光纤（FO）导丝直径（Diameter of hub opening or FO filament (mm)）、能量计记录的最小功率值（Minimum value (µW)）、最大功率值（Maximum value (µW)）、平均功率值（Mean value (µW)）、平均功率值的标准差（Standard deviation (µW)）、有效读数次数（Number of readings (n)）以及采集时长（Time (sec)）。本研究通过体外碟形样本实验，测定了三台装置对白假丝酵母（Candida albicans）ATCC 64124、粪肠球菌（Enterococcus faecalis）ATCC 29212、血链球菌（Streptococcus sanguinis）ATCC 10556、金黄色葡萄球菌（Staphylococcus aureus）ATCC 29213以及耐甲氧西林金黄色葡萄球菌（MRSA #7）的抗菌活性，相关原始数据详见表2；该数据集包含各组微生物的照射时长（Group (seconds)）、处理所用UVC LED装置（UVC LED treatment）、实验重复次数（Replication）、受试微生物种类（Microorganism）以及经UVC LED照射后存活的微生物菌落形成单位（CFU）数量（Viable CFU）。采用265 nm（22.5 mW）UVC LED装置，针对离体根管模型中的粪肠球菌ATCC 29212、白假丝酵母ATCC 64124、耐甲氧西林金黄色葡萄球菌（MRSA #7）以及金黄色葡萄球菌ATCC 29213的抗菌活性原始数据详见表3；该数据集包含分组类型（对照组或实验组，Group）、是否采用265 nm（22.5 mW）UVC LED装置进行照射（UVC LED treatment）、实验重复次数（Replication）、受试微生物种类（Microorganism）以及经265 nm（22.5 mW）装置照射后存活的微生物菌落形成单位（CFU）数量（Viable CFU）。