Genome-wide binding of MYCN protein in MYCN-amplified neuroblastoma cell lines

The MYCN locus is amplified in about half of high-risk neuroblastoma tumors. To identify genomic loci occupied by MYCN protein in the MYCN-amplified neuroblastoma cell lines NGP, Kelly and NB-1643, we performed chromatin immunoprecipitation coupled with Next-Generation Sequencing (ChIP-seq) using an anti-MYCN antibody. Overall design: Identification of MYCN occupancy throughout the genome of MYCN-amplified neuroblastoma cells.

MYCN基因座在约半数高风险神经母细胞瘤中发生扩增。为鉴定MYCN扩增型神经母细胞瘤细胞系NGP、Kelly及NB-1643中MYCN蛋白结合的基因组位点，我们使用抗MYCN抗体开展了染色质免疫沉淀结合下一代测序（ChIP-seq）实验。实验整体设计：鉴定MYCN扩增型神经母细胞瘤全基因组范围内的MYCN结合占据情况。