Effect of FSH on the gene expression profile of cumulus cells in vitro cultured as cumulus-oocyte complexes in presence of adenosine for 6 h. Effect of FSH on the gene expression profile of cumulus cells in vitro cultured as cumulus-oocyte complexes in presence of adenosine for 6 h

Changes in gene expression induced by FSH in cumulus cells treated with adenosine using Agilent EmbryoGENE microarray slides. Bovine cumulus-oocyte complexes in vitro-cultured for 6 h using adenosine. The effect of FSH was compared with control as non-treated cumulus-oocyte complexes. After culture, COC were were washed and cumulus cells were used for microarray analysis Overall design: Four pools of cumulus cells treated by FSH were hybridized against four pools of control cumulus cells in a dye-swap design.

本研究旨在探究经腺苷（adenosine）处理的卵丘细胞（cumulus cells）中，由促卵泡激素（Follicle-Stimulating Hormone，FSH）诱导的基因表达变化，实验采用安捷伦EmbryoGENE微阵列芯片（Agilent EmbryoGENE microarray slides）完成检测。实验对象为牛卵丘-卵母细胞复合体（cumulus-oocyte complexes，COCs），在添加腺苷的培养液中体外培养6小时。将施加FSH处理的实验组与未接受任何处理的卵丘-卵母细胞复合体对照组进行FSH生物学效应对比。培养结束后，对卵丘-卵母细胞复合体进行洗涤，分离得到的卵丘细胞用于后续微阵列分析。总体实验设计：采用染料交换（dye-swap）设计，将4份经FSH处理的卵丘细胞混合样本与4份对照卵丘细胞混合样本进行杂交。