Proposed ‘route’ taken by Edman sequencing to provide EPL001 from sSgII-70

The paper explores as meaningful anagrams MLKTGEKPV∙FK∙NNI (sSgII-14) and MKPLTGKVKEFNNI (EPL001). It is asserted that the residues in the three endogenous sSgII sequence modules were read in the wrong order by the Edman machine, instead of one by one from the N terminus, to give the EPL001 readout (SEQ ID NO: 3 of S1). This is the concept of Edman Nonsequentialism. Given the seven variable ovine sequences obtained (see S1), Edman Nonsequentialism has the status of an observation not a hypothesis.

本论文针对两个有意义的变位序列MLKTGEKPV∙FK∙NNI（sSgII-14）与MKPLTGKVKEFNNI（EPL001）展开研究。研究指出，埃德曼测序仪（Edman machine）在读取三个内源性sSgII序列模块的残基时出现了顺序错误——未按照从N端依次逐个读取的标准流程操作，最终得到了EPL001的测序结果（即补充材料S1中的SEQ ID NO:3）。这一概念即为埃德曼非顺序性（Edman Nonsequentialism）。鉴于已获取七个可变绵羊序列（详见S1），埃德曼非顺序性属于已观测到的实验结果，而非待验证的假说。